This study examined the validity and reliability of the Big Five personality traits scale in Malaysia. The sample of the study comprised 343 nurses in the health tourism hospitals. Administered on-site method was used for data collection. The respondents were required to rate their degree of agreement in the questionnaires with regard to their personality traits. Initial pilot test results showed an excellent internal reliability for each of the subscales. However, validity test extracted only four factors of the Big Five Inventory with factor loadings ranging from 0.573 to 0.803. The four factors were extraversion, conscientiousness, neuroticism and openness to experience. The reliability coefficients for all the extracted factors were above 0.7. Thus, the validated measures of the Big Five Inventory were deemed consistent and reliable throughout the study. Plausible reasons were stated in this study to explain the outcome of the factor analysis. Although many researchers recognized the Big Five Inventory as necessary and adequate to describe the structure of personality globally, this paper suggests that conducting validity and reliability test for Big Five Inventory is necessary when the study is conducted in countries with different cultural perspectives. In addition, it also offers suggestion to healthcare managers to identify their nurses' personality traits by using the validated measures. By knowing the nurses' personality traits, one can predict their work behaviors.