The verification/validation of a method is a necessary prerequisite before any activity in a medical biology laboratory. Also, it is important to remember the control of the risks associated with any dosing method as well as the impact of pre-analytical conditions on the post-analytical step. The objective of this work is to evaluate the analytical performance of serum and urinary glucose assay using the hexokinase enzymatic method of the manufacturer Abbott on the Alinity automaton of the same manufacturer. The verified parameters are reproducibility and repeatability, through the results of internal quality controls retrieved by creating procedures in the section of validation/verification of methods in our software: Big. The results of the performance evaluation in terms of reproducibility and repeatability demonstrate a perfect compliance of this method of serum and urine glucose determination by the Abbott kit on the Alinity PLC of the same manufacturer.

Gaucher disease is an autosomal recessive genetic metabolic disorder due to an inherited disorder of metabolism, resulting from glucocerebrosidase deficiency, resulting in the deposition of glucocerebroside and its components. The clinical symptoms are different depending on the type of the disease, but it is usually hepatosplenomegaly or damage to the central nervous system.Laboratory diagnosis can use routine analyzes such as ferritin, HDL, vitamin b12, folate, serum iron, but also specialized ones such as serum protein electrophoresis as well as immunofixation for the typing of immunoglobulins.Through this work, we will shed light on the interest of serum protein electrophoresis in the biological orientation of the biology diagnosis of Gaucher disease, and this through case illustrations.Confirmatory laboratory diagnosis is based on DNA analysis and / or enzymatic analysis of white blood cells, and by demonstrating a decrease in β-glucosidase activity...

Mitochondrial diseases, are the most frequent hereditary diseases of metabolism. They are characterized by a dysfunction of the respiratory chain, which results in an energy deficit.These are very heterogeneous diseases, with a very variable clinical presentation and often difficult diagnosis. They are due to the alteration of very diverse genes located either on mitochondrial DNA (mtDNA) or on the nuclear genome. Recent technological advances with exon sequencing have led to the discovery of many genes involved and to better understand the pathophysiological mechanisms of these diseases, which are essential for the development of specific treatments.The diagnostic approach consists in recognizing the disease in front of the clinical presentation, For clinical practice, the diagnostic approach of mitochondrial cytopathies would be more easy if the practitioner keeps in mind the most evocative clinical pictures and if he provides proof of the mitochondrial anomaly by biochemical, radiological and histopathological explorations. Only the highlighting of the causative gene makes it possible to affirm the diagnosis of mitochondrial disease. Genetic diagnosis allows genetic counseling, in order to support the prognosis, particularly pejorative for early-onset forms.

Background: Hypothyroidism is among the most common endocrine disease; it is often associated with dyslipidemia which increases cardiovascular risk. This study was conducted to study the prevalence and profile of dyslipidemia during primary hypothyroidism and justify screening for dyslipidemia during hypothyroidism.Methods: Retrospective study, in the biochemistry department CHU Ibn Rochd of Casablanca from 2019 to March 2020 involving all patients who received a lipid assessment and a TSH (Thyroid Stimulating Hormone) assay in the biochemistry laboratory. TSH was estimated by an immunoenzymatic method: chemiluminescence. The LDL-assayed (Low Density Lipoprotein), HDL (High Density Lipoprotein), TG (Triglycerides), CT (Total Cholesterol) parameters were estimated by an enzymatic colorimetric method, the LDL-calculated was calculated by the friedwald formula.Results: These were 1046 patients: 66.73% Women, 33.23% Men (sex ratio M / F = 0.49). The mean age was 41.66 years ± 13.86. The mean TSH value was 23.09 mIU / L. Dyslipidemia was noted in 91.78% of cases.The prevalences of, hypoHDLemia, hypercholesterolemia, hypertriglyceridemia, hyperLDLemia, were respectively: 82.12%; 32.5%; 23.32%; 10.8%.The correlation between TSH and LDL was negative (P> 0.05) while it was positive with TG (P> 0.05), HDL (P <0.05) and CT (P> 0.05).Conclusion: Hypothyroid patients show a significant increase in serum lipids. This atherogenic lipid profile should be systematically sought when monitoring patients with hypothyroidism to prevent cardiovascular complications.

The delocalized medical biology examinations are carried out outside the laboratory near the patient by staff outside the laboratory, which saves time in obtaining the results. These examinations are subject to the same regulations as examinations carried out in a medical biology laboratory and are the responsibility of medical biologists who retain responsibility for validating the results obtained.This development of the implantation of analytical devices of medical biology in the care units (outside the laboratory of the hospital) can be justified by several factors: medical emergencies, organizational constraints, the distance from the laboratory....In this work we tried to analyze the state of affairs of delocalized biology and to reveal the problems of delocalized biology of our ibn Rochd hospital center in Casablanca.

The Covid-19 outbreak is a global pandemic officially declared on March 12, 2020, with more than two million deaths. To assess the role of Procalcitonin as a biomarker of the specific inflammatory response and C-Réactive Protein as a stable and early biomarker of acute inflammation in pathogenesis and disease severity, we conducted a prospective descriptive study in the biochemistry laboratory of the Ibn Rochd University Of Casablanca Morocco in Covid-19 positive patients hospitalized in resuscitation. These biomarkers were abnormally increased in most of these patients. All of the patients who died had a positive admission CRP that would support a positive correlation between the increase in CRP and the severity of infection, without any correlation between CRP values in our study population with age or sex. The follow-up of the PRC could reflect its severity and should be used as a key monitoring indicator for Covid-19. PCT values at admission can predict the progression to a severe form of the disease. However, its synthesis is inhibited by interferon (INF) -γ, whose concentration increases during Coronavirus-2 infection.