[ Etude ethnobotanique et évaluation in vitro des activités antifongiques des extraits aqueux et hydro-éthanoliques de trois plantes médicinales ivoiriennes sur des germes fongiques responsables de mycoses superficielles en Côte d’Ivoire ]
KOUASSI Kouassi Germain1, Bolou Gbouhoury Eric-Kevin2, KONAN Gbé Kouakou N’Dri Ange3, YAPO Yomeh Cynthia Viviane4, AKA ANY-GRAH Adjoua Sandrine Armelle5, and ZIRIHI Guédé Noel6
1 Ecole Doctorale, Biologie-Environnement-Santé (ED-BES), Laboratoire des Milieux Naturels et Conservation de la Biodiversité, UFR Biosciences. Université Félix HOUPHOUËT- BOIGNY (UFHB), 22 BP 582 Abidjan 22, Côte d’Ivoire
2 National Center of Floristics, Félix Houphouët-Boigny University, Côte d’Ivoire
3 Laboratoire de Biologie et Santé, UFR Biosciences. Université Félix HOUPHOUËT- BOIGNY (UFHB), 22 BP 582 Abidjan 22, Côte d’Ivoire
4 Département des Sciences et Technologie, Université Alassane OUATTARA (UAO), BPV 18 Bouaké 01, Côte d’Ivoire
5 Laboratoire des Sciences du Médicament, Sciences Analytiques et Santé Publique, UFR de Sciences Pharmaceutiques et Biologiques (SPB), Université Félix OUPHOUËT- BOIGNY (UFHB), 22 BP 582 Abidjan 22, Côte d’Ivoire
6 Laboratoire de Botanique, Unité de Formation et de Recherche Biosciences, 22 BP 582 Abidjan 22, Université Felix Houphouët-Boigny, Côte d’Ivoire
Original language: French
Copyright © 2026 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Background: Following ethnobotanical surveys, Combretum paniculatum Vent (Combretaceae); Trema orientalis (L.) (Cannabaceae) and Duguetia staudtii (Diels & Engl) (Annonaceae) were the plants most cited and recommended in the treatment of various dermatoses, including superficial mycoses. Fungal infections, which are a frequent reason for consultation in dermatology, often have enormous consequences for victims, especially immunocompromised individuals. Objectives: To evaluate the in vitro antifungal activities of aqueous and hydro-ethanolic extracts of these plants on two dermatophytes (Trichophyton mentagrophytesR, Trichophyton rubrumR) and a yeast Candida albicansR 479 in order to confirm their use in traditional medicine and contribute to the fight against superficial mycoses which are on the rise in Ivory Coast. Methodology and results: Extracts from each plant were incorporated into Sabouraud agar using the double dilution method in tilted tubes. Dermatophytes (Trichophyton mentagrophytesR; Trichophyton rubrumR) and the yeast Candida albicansR 479 were sensitive to both aqueous and hydro-ethanolic extracts in 70% of the plants studied. However, it was the 70% hydro-ethanolic extracts that were the most active on all fungal strains with lower MICs (between 01.56 and 50 mg/ml); IC50 values ranging from (0.78 to 03.125 mg/ml). Conclusion and application of results: These results justify the traditional use of these medicinal plants and show that they would constitute a renewable and innovative source of therapeutic molecules that can be used to produce effective antifungals for the treatment of fungal dermatoses.
Author Keywords: ethnobotanical investigations, antifungal activities, medicinal plants, superficial mycoses.
KOUASSI Kouassi Germain1, Bolou Gbouhoury Eric-Kevin2, KONAN Gbé Kouakou N’Dri Ange3, YAPO Yomeh Cynthia Viviane4, AKA ANY-GRAH Adjoua Sandrine Armelle5, and ZIRIHI Guédé Noel6
1 Ecole Doctorale, Biologie-Environnement-Santé (ED-BES), Laboratoire des Milieux Naturels et Conservation de la Biodiversité, UFR Biosciences. Université Félix HOUPHOUËT- BOIGNY (UFHB), 22 BP 582 Abidjan 22, Côte d’Ivoire
2 National Center of Floristics, Félix Houphouët-Boigny University, Côte d’Ivoire
3 Laboratoire de Biologie et Santé, UFR Biosciences. Université Félix HOUPHOUËT- BOIGNY (UFHB), 22 BP 582 Abidjan 22, Côte d’Ivoire
4 Département des Sciences et Technologie, Université Alassane OUATTARA (UAO), BPV 18 Bouaké 01, Côte d’Ivoire
5 Laboratoire des Sciences du Médicament, Sciences Analytiques et Santé Publique, UFR de Sciences Pharmaceutiques et Biologiques (SPB), Université Félix OUPHOUËT- BOIGNY (UFHB), 22 BP 582 Abidjan 22, Côte d’Ivoire
6 Laboratoire de Botanique, Unité de Formation et de Recherche Biosciences, 22 BP 582 Abidjan 22, Université Felix Houphouët-Boigny, Côte d’Ivoire
Original language: French
Copyright © 2026 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Background: Following ethnobotanical surveys, Combretum paniculatum Vent (Combretaceae); Trema orientalis (L.) (Cannabaceae) and Duguetia staudtii (Diels & Engl) (Annonaceae) were the plants most cited and recommended in the treatment of various dermatoses, including superficial mycoses. Fungal infections, which are a frequent reason for consultation in dermatology, often have enormous consequences for victims, especially immunocompromised individuals. Objectives: To evaluate the in vitro antifungal activities of aqueous and hydro-ethanolic extracts of these plants on two dermatophytes (Trichophyton mentagrophytesR, Trichophyton rubrumR) and a yeast Candida albicansR 479 in order to confirm their use in traditional medicine and contribute to the fight against superficial mycoses which are on the rise in Ivory Coast. Methodology and results: Extracts from each plant were incorporated into Sabouraud agar using the double dilution method in tilted tubes. Dermatophytes (Trichophyton mentagrophytesR; Trichophyton rubrumR) and the yeast Candida albicansR 479 were sensitive to both aqueous and hydro-ethanolic extracts in 70% of the plants studied. However, it was the 70% hydro-ethanolic extracts that were the most active on all fungal strains with lower MICs (between 01.56 and 50 mg/ml); IC50 values ranging from (0.78 to 03.125 mg/ml). Conclusion and application of results: These results justify the traditional use of these medicinal plants and show that they would constitute a renewable and innovative source of therapeutic molecules that can be used to produce effective antifungals for the treatment of fungal dermatoses.
Author Keywords: ethnobotanical investigations, antifungal activities, medicinal plants, superficial mycoses.
Abstract: (french)
Contexte: Suite à des enquêtes ethnobotaniques, Combretum paniculatum Vent (Combretaceae); Trema orientalis (L.) (Cannabaceae) et Duguetia staudtii (Diels & Engl) (Annonaceae) ont été les plantes, les plus citées et recommandées dans le traitement de diverses dermatoses, dont les mycoses superficielles. Constituant un motif fréquent de consultation en dermatologie, les mycoses présentent souvent d’énormes conséquences chez les victimes, surtout les immunodéprimés. Objectifs: Evaluer les activités antifongiques in vitro des extraits aqueux et hydro-éthanoliques de ces plantes sur deux dermatophytes (Trichophyton mentagrophytesR, Trichophyton rubrumR) et une levure Candida albicansR 479 afin de confirmer leur usage en médecine traditionnelle et contribuer à la lutte contre les mycoses superficielles à forte recrudescence en Côte d’Ivoire. Méthodologie et résultats: Les extraits de chaque plante ont été incorporés à la gélose Sabouraud Agar selon la méthode de double dilution en tubes penchés. Les dermatophytes (Trichophyton mentagrophytesR; Trichophyton rubrumR) et la levure Candida albicansR 479 ont été sensibles aux extraits aqueux aussi bien que ceux hydro-éthanoliques 70% des plantes étudiées. Cependant, ce sont les extraits hydro-éthanoliques 70% qui ont été les plus actifs sur toutes les souches fongiques avec des CMI plus faibles (compris entre 01,56 et 50 mg/ml); des valeurs de la CI50 allant de (0,78 à 03,125.mg/ml). Conclusion et application des résultats: Ces résultats permettent de justifier l’utilisation traditionnelle de ces plantes médicinales et montrent qu’elles constitueraient une source renouvelable et innovante des molécules thérapeutiques pouvant servir à la production d’antifongiques efficaces pour le traitement des dermatoses fongiques.
Author Keywords: investigations ethnobotaniques, activités antifongiques, plantes médicinales, mycoses superficielles.
