[ Induction de cals des explants de trois provenances de Karité (Vitellaria paradoxa) au laboratoire d’Agro physio-génétique et de biotechnologies végétales de l’IPR/IFRA de Katibougou ]
Moussa Dramane Traoré1, Moussa Abdoulaye2, Aly Kansaye3, and Moussa Karembé4
1 Laboratoire d’Agro-physio-génétique et de biotechnologies végétales, IPR, IFRA, Katibougou, Mali
2 Laboratoire d’Agro-physio-génétique et de biotechnologies végétales, IPR, IFRA, Katibougou, Mali
3 Laboratoire d’Agro-physio-génétique et de biotechnologies végétales, IPR, IFRA, Katibougou, Mali
4 Laboratoire d’Ecologie Tropicale, Faculté des Sciences et Techniques, Bamako, Mali
Original language: French
Copyright © 2026 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Shea is a species native to the Sudanian-Sahelian zone. This species is confronted by climate change and human activity. Unfortunately, young plants do not have time to grow due to their very slow growth rate in their natural environment. An in vitro production study at the IPR/IFRA Laboratory of Agro-physio-genetics and Plant Biotechnology is underway for the improvement of this species. A completely randomized design was used. The plant material consisted of three provenances of leaf explants. The parameters observed were contamination, the rate of explants initiated, the rate of explants forming calluses, callus expression, weight, callus diameter in light and dark conditions, and callus regeneration. The STATITCF software was used for data analysis. The best disinfection rate was observed with the T3 test, with 2.5% contamination. The survival rate showed no significant difference (P=0.0934). However, a highly significant difference was found between the sources, with 75.35% for the Siby explants. For callus induction, a highly significant difference (P=0.0000) was observed. Regarding provenance, the analysis of variance showed no significant difference (P=0.9557). Different callus shapes and appearances were observed under conditions of light and continuous darkness Analysis showed a highly significant difference (P=0.0119) in callus survival rate between expression media. Under light culture conditions, the diameter and weight of the calluses showed no significant difference (P=0.3101). However, under continuous darkness, the analysis of variance revealed a highly significant difference (P=0.0018) of 1 cm and 1.39 g, respectively.
Author Keywords: Mali, photoperiod, culture, regeneration, hormone.
Moussa Dramane Traoré1, Moussa Abdoulaye2, Aly Kansaye3, and Moussa Karembé4
1 Laboratoire d’Agro-physio-génétique et de biotechnologies végétales, IPR, IFRA, Katibougou, Mali
2 Laboratoire d’Agro-physio-génétique et de biotechnologies végétales, IPR, IFRA, Katibougou, Mali
3 Laboratoire d’Agro-physio-génétique et de biotechnologies végétales, IPR, IFRA, Katibougou, Mali
4 Laboratoire d’Ecologie Tropicale, Faculté des Sciences et Techniques, Bamako, Mali
Original language: French
Copyright © 2026 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Shea is a species native to the Sudanian-Sahelian zone. This species is confronted by climate change and human activity. Unfortunately, young plants do not have time to grow due to their very slow growth rate in their natural environment. An in vitro production study at the IPR/IFRA Laboratory of Agro-physio-genetics and Plant Biotechnology is underway for the improvement of this species. A completely randomized design was used. The plant material consisted of three provenances of leaf explants. The parameters observed were contamination, the rate of explants initiated, the rate of explants forming calluses, callus expression, weight, callus diameter in light and dark conditions, and callus regeneration. The STATITCF software was used for data analysis. The best disinfection rate was observed with the T3 test, with 2.5% contamination. The survival rate showed no significant difference (P=0.0934). However, a highly significant difference was found between the sources, with 75.35% for the Siby explants. For callus induction, a highly significant difference (P=0.0000) was observed. Regarding provenance, the analysis of variance showed no significant difference (P=0.9557). Different callus shapes and appearances were observed under conditions of light and continuous darkness Analysis showed a highly significant difference (P=0.0119) in callus survival rate between expression media. Under light culture conditions, the diameter and weight of the calluses showed no significant difference (P=0.3101). However, under continuous darkness, the analysis of variance revealed a highly significant difference (P=0.0018) of 1 cm and 1.39 g, respectively.
Author Keywords: Mali, photoperiod, culture, regeneration, hormone.
Abstract: (french)
Le karité est une espèce de la zone soudano-sahélienne. Cette espèce est confrontée aux aléas climatiques et des actions anthropiques. Les jeunes plants n’ont malheureusement pas le temps de grandir à cause de la croissance très lente dans leurs milieux naturelle. Des recherches de production in vitro au laboratoire d’Agro-physio-génétique et de biotechnologies végétales de l’IPR/IFRA est en cours pour l’amélioration de cette espèce. Un dispositif en randominisation totale a été adopté. Le matériel végétal était constitué de trois provenances d’explants foliaires. Les paramètres observés ont été contamination, le taux d’explants initiés, le taux d’explants formant des cals, l’expression des cals, le poids, le diamètre radial des cals en condition de lumière et d’obscurité, la régénération des cals. Le logiciel STATITCF a servi l’analyse des données. La meilleure désinfection a lieu avec le test T3 avec 2,5% de contamination. Le taux de survie n’a pas montré de différence significative (P=0.0934). Par contre une différence hautement significative a lieu entre les provenances avec 75,35% pour les explants de Siby. Pour l’induction de cals, une différence hautement significative (P=0.0000) a été observée. Quant aux provenances, l’analyse de variance n’a pas montré de différence significative (P=0.9557). Différentes formes et aspects des cals ont été observés dans les conditions de 16h/8 de lumière et d’obscurité continue. L’analyse a montré une différence hautement significative (P=0.0119) du taux de survie des cals formés. En condition de culture de 16h/8 de lumière, le diamètre radial et le poids des cals n’ont pas montré de différence significative (P=0.3101). Cependant en obscurité continue, l’analyse de variance a donné une différence hautement significative (P=0.0018) respectivement 1cm et 1,39g.
Author Keywords: Mali, photopériode, culture, régénération, hormone.
