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International Journal of Innovation and Applied Studies
ISSN: 2028-9324     CODEN: IJIABO     OCLC Number: 828807274     ZDB-ID: 2703985-7
 
 
Thursday 27 June 2019

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Development and validation of a quantitative determination method of ethanol in Human Plasma: Experience of Laboratory of Toxicology and Pharmacology, Moroccan Poison Control and Pharmacovigilance Center


Volume 7, Issue 2, August 2014, Pages 655–661

 Development and validation of a quantitative determination method of ethanol in Human Plasma: Experience of Laboratory of Toxicology and Pharmacology, Moroccan Poison Control and Pharmacovigilance Center

Mostafa Khaya1, Mouncef Idrissi2, Rachida Soulaymani-Bencheikh3, Mohammed Ghandi4, Naima Ait Daoud5, Bouchra Birich6, Seloua Elmrabeh7, Abderrahim El Hourch8, and Aicha Guessous9

1 Faculty of science, Mohammed V University – Agdal, B.P. 1014, Rabat, Morocco
2 Poison Control and Pharmacovigilance Centre of Morocco, B.P. 6671, Rabat, Morocco
3 Faculté de Médecine et de Pharmacie, Université Mohammed V - Souissi, Rabat, Morocco
4 Poison Control and Pharmacovigilance Centre of Morocco, B.P. 6671, Rabat, Morocco
5 Poison Control and Pharmacovigilance Centre of Morocco, B.P. 6671, Rabat, Morocco
6 Faculty of science, Mohammed V university–Agdal, B.P. 1014, Rabat, Morocco
7 Poison Control and Pharmacovigilance Centre of Morocco, B.P. 6671, Rabat, Morocco
8 Faculty of science, Mohammed V university–Agdal, B.P. 1014, Rabat, Morocco
9 Faculty of science, Mohammed V university–Agdal, B.P. 1014, Rabat, Morocco

Original language: English

Received 20 June 2014

Copyright © 2014 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract


Acute intoxication and chronic ethanol is recognized by specific symptoms and needs to be confirmed by blood or plasma ethanol dosage. Although, the aim of our paper is to develop and validate a method in the order to determinate the amount of blood ethanol, using gas chromatography with flame ionization detection (FID), after a Headspace sampling. We used butanol as an internal standard, our method is specific and linear at the range amount from 0,1g/L to 5 g/L. the coefficient of correlation was 0.9998. Using the quality control from standard solution we determined the coefficient of variation and recovery percent, compared to the theoretical amount (25%; 50% and 75% of the maximum amount). The coefficient of variation was within 4.046%; 9.682% and 3.553 % at concentration of 0.3; 2.5 and 4 g/L, respectively. The limit of detection was 0.063 g/L. In the same conditions, limit of quantification was 0.085 g/L. The storage's and transport's samples were at +4

Author Keywords: Ethanol, plasma, blood, Morocco, chromatography, Headspace, validation.


How to Cite this Article


Mostafa Khaya, Mouncef Idrissi, Rachida Soulaymani-Bencheikh, Mohammed Ghandi, Naima Ait Daoud, Bouchra Birich, Seloua Elmrabeh, Abderrahim El Hourch, and Aicha Guessous, “Development and validation of a quantitative determination method of ethanol in Human Plasma: Experience of Laboratory of Toxicology and Pharmacology, Moroccan Poison Control and Pharmacovigilance Center,” International Journal of Innovation and Applied Studies, vol. 7, no. 2, pp. 655–661, August 2014.