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International Journal of Innovation and Applied Studies
ISSN: 2028-9324     CODEN: IJIABO     OCLC Number: 828807274     ZDB-ID: 2703985-7
 
 
Wednesday 22 May 2019

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In vitro suppression of the crown gall (Agrobacterium tumefaciens) by compost extracts bacteria


Volume 7, Issue 2, August 2014, Pages 617–623

 In vitro suppression of the crown gall (Agrobacterium tumefaciens) by compost extracts bacteria

Amel Kerkeni1, Ahmed Marichali Mze2, Saloua Ouerghemmi3, Sana Dallai4, Saoussen Benzarti5, and Mohamed Ben Khedher6

1 Research Laboratory of Agricultural Production Systems and Sustainable Development, College of Agriculture, the University of Carthage, 1121 Mograne, Zaghouan, Tunisia
2 Research Laboratory of Agricultural Production Systems and Sustainable Development, College of Agriculture, the University of Carthage, 1121 Mograne, Zaghouan, Tunisia
3 Research Laboratory of Agricultural Production Systems and Sustainable Development, College of Agriculture, the University of Carthage, 1121 Mograne, Zaghouan, Tunisia
4 Research Laboratory of Agricultural Production Systems and Sustainable Development, College of Agriculture, the University of Carthage, 1121 Mograne, Zaghouan, Tunisia
5 Research Laboratory of Agricultural Production Systems and Sustainable Development, College of Agriculture, the University of Carthage, 1121 Mograne, Zaghouan, Tunisia
6 Department of agronomy, the University of Sousse, Chatt Mariem, 4042, Sousse, Tunisia

Original language: English

Received 21 June 2014

Copyright © 2014 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract


Nine kinds of compost extracts were tested primarily for their efficiency, in vitro, against the causal agent of crown gall Agrobacterium tumefaciens (strain C58). The most efficient extracts were then selected and bacteria contained in these extracts were isolated. Twenty-seven isolates bacteria were obtained and investigated in vitro with the objective of selecting efficient antagonists against crown gall disease. The bacterial activity is compared to the reference antagonist Agrobacterium rhizogenes K84 by the double layer method.
In vitro analyzing the antagonistic activity revealed that, after incubation at 27

Author Keywords: compost extracts, antagonists, Agrobacterium rhizogenes K84, Agrobacterium tumefaciens, inhibition, control.


How to Cite this Article


Amel Kerkeni, Ahmed Marichali Mze, Saloua Ouerghemmi, Sana Dallai, Saoussen Benzarti, and Mohamed Ben Khedher, “In vitro suppression of the crown gall (Agrobacterium tumefaciens) by compost extracts bacteria,” International Journal of Innovation and Applied Studies, vol. 7, no. 2, pp. 617–623, August 2014.