International Journal of Innovation and Applied Studies
ISSN: 2028-9324     CODEN: IJIABO     OCLC Number: 828807274     ZDB-ID: 2703985-7
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Using of Iraqi probiotic to detoxify Patulin in albino mice

Volume 11, Issue 2, May 2015, Pages 282–290

 Using of Iraqi probiotic to detoxify Patulin in albino mice

Shatha Ali Shafiq1

1 Department of Biology, College of Science, Mustansiriyah University, Baghdad, Iraq

Original language: English

Received 15 March 2015

Copyright © 2015 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The present study was to evaluate the histological and biochemical toxic effects of Patulin mycotoxin on liver and kidney tissues of males white mice that extracted from the fungal isolate Penicillium expansum at concentration (3.5 mg / kg body weight) given single and repeated doses of Patulin orally administration and the second aspect of this study using of Iraqi probiotic (local product) as detoxifying agent of Patulin. Mice were divided into four groups five mice for each group, taking into consideration liver and kidney weights. Groups of mice were treated as follows. T1: given the toxin once and sacrificed after two days. T2: given the toxin twice for one week. T3: given the toxin twice a week for two weeks. T4: The last group was treated by Patulin for five days orally with addition Iraqi probiotic (2 %) w/ v in drinking water for fifteen days. Each treated group has its corresponding control which received 1% Dimethyl Sulphoxide (DMSO). The results of the treatment of mice males Patulin of 3.5 mg / kg, in three groups (T1,T2,T3) had caused histological changes in the liver tissue represented by degeneration and necrosis in hepatocytes and had increased with increasing of repeated doses of toxin especially at T3 group that it revealed histological changes represented by congestion of central vein with inflammatory cells in their lumen, lymphocytes infiltration and hemorrhage especially near portal space . The results of liver enzymes showed significant decrease in (Aspartate Aminotransferase) AST, (Alanine Aminotransferase) ALT in treated mice with Patulin (repeated doses) which were involved T2 and T3 group compared with the other groups , while the results of enzymatic activity of (Alkaline phosphatase) ALP showed non-significant decrease in all groups . No obvious significant difference in the concentration of Creatinine in all groups, while urea concentration recorded significant decrease in T2 and T3 groups as compared with T1 and T4 groups. Patulin exhibits Histopathological changes coincided with biochemical changes observed in experiments on male mice and the effect of Patulin depended on time of exposure. The other part of the present study was to investigate the biological degradation of Patulin represented by T4 group using by Iraqi probiotic 2% (w/v) which had an active influence to protect the liver and kidney tissues from the toxic effect of Patulin.

Author Keywords: Patulin, Iraqi probiotic, Histological changes, Biochemical tests.

How to Cite this Article

Shatha Ali Shafiq, “Using of Iraqi probiotic to detoxify Patulin in albino mice,” International Journal of Innovation and Applied Studies, vol. 11, no. 2, pp. 282–290, May 2015.