Volume 47, Issue 2, December 2025, Pages 138–146
Farhana Ilyas Khan1 and Samina Malik2
1 Department of Botany, University of Agriculture, Faisalabad, Punjab 38000, Pakistan
2 Department of Botany, University of Agriculture, Faisalabad, Punjab 38000, Pakistan
Original language: English
Copyright © 2025 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Ascorbic acid (Vitamin C) is a growth regulator that stimulates plant growth and development. First, a preliminary experiment was performed to find the optimal Vitamin C dosage for maize plants. A single genotype of maize, Golden, was grown in sand under pot conditions. Hoagland’s nutrient solution was applied for seven days. Four doses of Vitamin C (i.e., 0.5, 1, 1.5and 2 mM) were applied as foliar spray on maize seedlings for two weeks. Then, in the main experiment, two genotypes of maize (Zea mays L.) namely Golden and Agaiti 2000 were grown in soil in a wire house at the Botanical Garden, the University of Agriculture in Faisalabad (Pakistan). Based on the preliminary results, the optimal dose (2 mM) of ascorbic acid was applied exogenously as foliar spray on leaves of both genotypes every third day for five weeks at seedling stage (3 leaf stage). Findings were noted both qualitatively and quantitatively in terms of different physiological and growth parameters. In addition, COSTAT software was employed to perform statistical analysis of quantitative readings for all parameters using analysis of variance (ANOVA) technique. It was observed that treated plants showed improved health and growth compared to non-treated plants with higher contents of physiological variables including chlorophyll, carotenoids, soluble carbohydrates, and minerals etc. and increased leaves, roots and shoots sizes and biomass. Moreover, Agaiti 2000 genotype was observed to show better results than genotype golden.
Author Keywords: Maize, ascorbic acid (AsA), vitamin C, foliar spray, growth, statistical analysis.
Farhana Ilyas Khan1 and Samina Malik2
1 Department of Botany, University of Agriculture, Faisalabad, Punjab 38000, Pakistan
2 Department of Botany, University of Agriculture, Faisalabad, Punjab 38000, Pakistan
Original language: English
Copyright © 2025 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Ascorbic acid (Vitamin C) is a growth regulator that stimulates plant growth and development. First, a preliminary experiment was performed to find the optimal Vitamin C dosage for maize plants. A single genotype of maize, Golden, was grown in sand under pot conditions. Hoagland’s nutrient solution was applied for seven days. Four doses of Vitamin C (i.e., 0.5, 1, 1.5and 2 mM) were applied as foliar spray on maize seedlings for two weeks. Then, in the main experiment, two genotypes of maize (Zea mays L.) namely Golden and Agaiti 2000 were grown in soil in a wire house at the Botanical Garden, the University of Agriculture in Faisalabad (Pakistan). Based on the preliminary results, the optimal dose (2 mM) of ascorbic acid was applied exogenously as foliar spray on leaves of both genotypes every third day for five weeks at seedling stage (3 leaf stage). Findings were noted both qualitatively and quantitatively in terms of different physiological and growth parameters. In addition, COSTAT software was employed to perform statistical analysis of quantitative readings for all parameters using analysis of variance (ANOVA) technique. It was observed that treated plants showed improved health and growth compared to non-treated plants with higher contents of physiological variables including chlorophyll, carotenoids, soluble carbohydrates, and minerals etc. and increased leaves, roots and shoots sizes and biomass. Moreover, Agaiti 2000 genotype was observed to show better results than genotype golden.
Author Keywords: Maize, ascorbic acid (AsA), vitamin C, foliar spray, growth, statistical analysis.
How to Cite this Article
Farhana Ilyas Khan and Samina Malik, “Effect of Exogenously Applied Vitamin C (Ascorbic Acid) as Foliar Spray on the Growth of Maize (Zea mays L.),” International Journal of Innovation and Applied Studies, vol. 47, no. 2, pp. 138–146, December 2025.
