Volume 46, Issue 3, September 2025, Pages 620–627
Ibourahema Coulibaly1, Condé Fatoumata2, Kalpy Julien Coulibaly3, Konaté Ibrahim4, and Daouda Koné5
1 Lecturer, Teacher-Researcher, Agrovalorization Laboratory, Department of Biochemistry-Microbiology, UFR Agroforestry, Jean Lorougnon Guédé University, BP 150 Daloa, Côte d'Ivoire
2 Researcher, Agrovalorization Laboratory, Department of Biochemistry-Microbiology, UFR Agroforestry, Jean Lorougnon Guédé University, BP 150 Daloa, Côte d'Ivoire
3 Associate Professor, Physician, University Hospital Center (CHU) of Cocody, Environmental Chemistry and Microbiology Unit, Institut Pasteur de Côte d’Ivoire, BP 490 Abidjan 01, Côte d'Ivoire
4 Full Professor, Teacher-Researcher, Laboratory of Agrovalorization, Department of Biochemistry-Microbiology, UFR Agroforestry, Jean Lorougnon Guédé University, BP 150 Daloa, Côte d'Ivoire
5 Full Professor, Teacher-Researcher, Laboratory of Plant Physiology, Department of Biology, UFR of Bioscience, Abidjan, Felix Houphouët Boigny University, BP V34 Abidjan 01, Côte d'Ivoire
Original language: English
Copyright © 2025 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The research presented in this memory was carried out at the Institut Pasteur in Côte d’Ivoire and carried out on E. coli and Salmonella spp. The objective of this study was to isolate E. coli and Salmonella spp. strains resistant to antibiotics from wastewater from the Port-Bouët slaughterhouse. From September 2017 to March 2018, 17 strains of E. coli and 7 Salmonella spp. were isolated in 63 samples of wastewater collected at the slaughterhouse of Port-Bouët. Their identification was performed according to conventional bacteriological tests. An antibiogram according to the disk diffusion method was carried out for 21 antibiotics commonly used in human and veterinary medicine. The prevalence observed was 26.98% for E coli strains. and 11.11% for Salmonella spp. All strains of E. coli showed complete (100%) resistance to 3rd generation cephalosporins, aztreonam, amocixillin + clavulanic acid, and ampicillin. However, family resistance rates of quinolones, aminoglycosides, sulfonamides and cyclins remain very high. The sensitivity of E. coli to imipenem and amikacin was 100%. Salmonella spp. strains, on the other hand, were resistant only to beta-lactam at lower levels compared to E. coli strains. coli. In-depth studies are needed to determine the resistance mechanisms of these bacteria.
Author Keywords: Escherichia coli, Salmonella spp., beta-lactam, quinolones, sulfonamides, aminoglycosides, cyclins, antibiogram.
Ibourahema Coulibaly1, Condé Fatoumata2, Kalpy Julien Coulibaly3, Konaté Ibrahim4, and Daouda Koné5
1 Lecturer, Teacher-Researcher, Agrovalorization Laboratory, Department of Biochemistry-Microbiology, UFR Agroforestry, Jean Lorougnon Guédé University, BP 150 Daloa, Côte d'Ivoire
2 Researcher, Agrovalorization Laboratory, Department of Biochemistry-Microbiology, UFR Agroforestry, Jean Lorougnon Guédé University, BP 150 Daloa, Côte d'Ivoire
3 Associate Professor, Physician, University Hospital Center (CHU) of Cocody, Environmental Chemistry and Microbiology Unit, Institut Pasteur de Côte d’Ivoire, BP 490 Abidjan 01, Côte d'Ivoire
4 Full Professor, Teacher-Researcher, Laboratory of Agrovalorization, Department of Biochemistry-Microbiology, UFR Agroforestry, Jean Lorougnon Guédé University, BP 150 Daloa, Côte d'Ivoire
5 Full Professor, Teacher-Researcher, Laboratory of Plant Physiology, Department of Biology, UFR of Bioscience, Abidjan, Felix Houphouët Boigny University, BP V34 Abidjan 01, Côte d'Ivoire
Original language: English
Copyright © 2025 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
The research presented in this memory was carried out at the Institut Pasteur in Côte d’Ivoire and carried out on E. coli and Salmonella spp. The objective of this study was to isolate E. coli and Salmonella spp. strains resistant to antibiotics from wastewater from the Port-Bouët slaughterhouse. From September 2017 to March 2018, 17 strains of E. coli and 7 Salmonella spp. were isolated in 63 samples of wastewater collected at the slaughterhouse of Port-Bouët. Their identification was performed according to conventional bacteriological tests. An antibiogram according to the disk diffusion method was carried out for 21 antibiotics commonly used in human and veterinary medicine. The prevalence observed was 26.98% for E coli strains. and 11.11% for Salmonella spp. All strains of E. coli showed complete (100%) resistance to 3rd generation cephalosporins, aztreonam, amocixillin + clavulanic acid, and ampicillin. However, family resistance rates of quinolones, aminoglycosides, sulfonamides and cyclins remain very high. The sensitivity of E. coli to imipenem and amikacin was 100%. Salmonella spp. strains, on the other hand, were resistant only to beta-lactam at lower levels compared to E. coli strains. coli. In-depth studies are needed to determine the resistance mechanisms of these bacteria.
Author Keywords: Escherichia coli, Salmonella spp., beta-lactam, quinolones, sulfonamides, aminoglycosides, cyclins, antibiogram.
How to Cite this Article
Ibourahema Coulibaly, Condé Fatoumata, Kalpy Julien Coulibaly, Konaté Ibrahim, and Daouda Koné, “Detection of antibiotic-resistant Escherichia coli and Salmonella spp. in wastewater from a former slaughterhouse in Ivory Coast,” International Journal of Innovation and Applied Studies, vol. 46, no. 3, pp. 620–627, September 2025.
